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Update README.org
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@@ -230,8 +230,8 @@ results in repeated runs. In particular, one feature is prone to generate
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differences when employed in proovread's iterative strategy: for performance
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reasons bwa encodes nucleotides using 2 bits only, meaning bwa only has a
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four letter alphabet =[ATGC]=. Other bases, including =NNNN= stretches used
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for masking by proovread, are converted into random =[ATGC]= strings. This
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has the most severe effect on alignments at the margins of masked regions:
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for masking by proovread, are converted into random =[ATGC]= strings. This,
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in particular, effects alignments at the margins of masked regions:
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#+BEGIN_EXAMPLE
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orig | ATGAATTGGTTAATCTGC
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masked | ATGAATTGGTNNNNNNNN
@@ -247,15 +247,14 @@ has the most severe effect on alignments at the margins of masked regions:
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#+END_EXAMPLE
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***** sorting with threshold
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whenever there are decisions to make based on some sort of sorted list in
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combination with fixed amount of items to keep/remove, things get
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non-deterministic, if different items can have identical values in their sorting
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fields. In proovread, this for example affects filtering of "best alignments" in
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bins (local context).
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Whenever there are decisions to make for sorted list in combination with fixed
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amount of items to keep/remove, things get non-deterministic if identical values in sorting
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fields occur. In proovread, this for example affects filtering of "best alignments" in
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bins (localized scoring context).
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***** consensus calling
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Whenever there is a 50-50 ratio for a base call, the resulting base is randomly
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chosen, to minimize particular biases.
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50-50 ratios in base calling will result in one randomly
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chosen alternative, minimizing a particular bias.
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** Algorithm and Implementation
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Algorithm and Implementation are described in detail in the [[http://dx.doi.org/10.1093/bioinformatics/btu392][proovread]] paper.

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