-
Notifications
You must be signed in to change notification settings - Fork 55
Expand file tree
/
Copy path_def.py
More file actions
947 lines (768 loc) · 26.1 KB
/
Copy path_def.py
File metadata and controls
947 lines (768 loc) · 26.1 KB
1
2
3
4
5
6
7
8
9
10
11
12
13
14
15
16
17
18
19
20
21
22
23
24
25
26
27
28
29
30
31
32
33
34
35
36
37
38
39
40
41
42
43
44
45
46
47
48
49
50
51
52
53
54
55
56
57
58
59
60
61
62
63
64
65
66
67
68
69
70
71
72
73
74
75
76
77
78
79
80
81
82
83
84
85
86
87
88
89
90
91
92
93
94
95
96
97
98
99
100
101
102
103
104
105
106
107
108
109
110
111
112
113
114
115
116
117
118
119
120
121
122
123
124
125
126
127
128
129
130
131
132
133
134
135
136
137
138
139
140
141
142
143
144
145
146
147
148
149
150
151
152
153
154
155
156
157
158
159
160
161
162
163
164
165
166
167
168
169
170
171
172
173
174
175
176
177
178
179
180
181
182
183
184
185
186
187
188
189
190
191
192
193
194
195
196
197
198
199
200
201
202
203
204
205
206
207
208
209
210
211
212
213
214
215
216
217
218
219
220
221
222
223
224
225
226
227
228
229
230
231
232
233
234
235
236
237
238
239
240
241
242
243
244
245
246
247
248
249
250
251
252
253
254
255
256
257
258
259
260
261
262
263
264
265
266
267
268
269
270
271
272
273
274
275
276
277
278
279
280
281
282
283
284
285
286
287
288
289
290
291
292
293
294
295
296
297
298
299
300
301
302
303
304
305
306
307
308
309
310
311
312
313
314
315
316
317
318
319
320
321
322
323
324
325
326
327
328
329
330
331
332
333
334
335
336
337
338
339
340
341
342
343
344
345
346
347
348
349
350
351
352
353
354
355
356
357
358
359
360
361
362
363
364
365
366
367
368
369
370
371
372
373
374
375
376
377
378
379
380
381
382
383
384
385
386
387
388
389
390
391
392
393
394
395
396
397
398
399
400
401
402
403
404
405
406
407
408
409
410
411
412
413
414
415
416
417
418
419
420
421
422
423
424
425
426
427
428
429
430
431
432
433
434
435
436
437
438
439
440
441
442
443
444
445
446
447
448
449
450
451
452
453
454
455
456
457
458
459
460
461
462
463
464
465
466
467
468
469
470
471
472
473
474
475
476
477
478
479
480
481
482
483
484
485
486
487
488
489
490
491
492
493
494
495
496
497
498
499
500
501
502
503
504
505
506
507
508
509
510
511
512
513
514
515
516
517
518
519
520
521
522
523
524
525
526
527
528
529
530
531
532
533
534
535
536
537
538
539
540
541
542
543
544
545
546
547
548
549
550
551
552
553
554
555
556
557
558
559
560
561
562
563
564
565
566
567
568
569
570
571
572
573
574
575
576
577
578
579
580
581
582
583
584
585
586
587
588
589
590
591
592
593
594
595
596
597
598
599
600
601
602
603
604
605
606
607
608
609
610
611
612
613
614
615
616
617
618
619
620
621
622
623
624
625
626
627
628
629
630
631
632
633
634
635
636
637
638
639
640
641
642
643
644
645
646
647
648
649
650
651
652
653
654
655
656
657
658
659
660
661
662
663
664
665
666
667
668
669
670
671
672
673
674
675
676
677
678
679
680
681
682
683
684
685
686
687
688
689
690
691
692
693
694
695
696
697
698
699
700
701
702
703
704
705
706
707
708
709
710
711
712
713
714
715
716
717
718
719
720
721
722
723
724
725
726
727
728
729
730
731
732
733
734
735
736
737
738
739
740
741
742
743
744
745
746
747
748
749
750
751
752
753
754
755
756
757
758
759
760
761
762
763
764
765
766
767
768
769
770
771
772
773
774
775
776
777
778
779
780
781
782
783
784
785
786
787
788
789
790
791
792
793
794
795
796
797
798
799
800
801
802
803
804
805
806
807
808
809
810
811
812
813
814
815
816
817
818
819
820
821
822
823
824
825
826
827
828
829
830
831
832
833
834
835
836
837
838
839
840
841
842
843
844
845
846
847
848
849
850
851
852
853
854
855
856
857
858
859
860
861
862
863
864
865
866
867
868
869
870
871
872
873
874
875
876
877
878
879
880
881
882
883
884
885
886
887
888
889
890
891
892
893
894
895
896
897
898
899
900
901
902
903
904
905
906
907
908
909
910
911
912
913
914
915
916
917
918
919
920
921
922
923
924
925
926
927
928
929
930
931
932
933
934
935
936
937
938
939
940
941
942
943
944
945
946
947
import os
import sys
import glob
from pathlib import Path
from configparser import ConfigParser
from typing import Union
import json
import csv
import squid.logging
from enum import Enum, auto
log = squid.logging.get_logger(__name__)
def conf_attribute_reader(string_value):
"""
:brief: standardized way for reading config entries
that are strings, in priority order
None -> bool -> dict/list (via json) -> int -> float -> string
REMEMBER TO ENCLOSE PROPERTY NAMES IN LISTS/DICTS IN
DOUBLE QUOTES
"""
actualvalue = str(string_value).strip()
try:
if str(actualvalue) == "None":
return None
except:
pass
try:
if str(actualvalue) == "True" or str(actualvalue) == "true":
return True
if str(actualvalue) == "False" or str(actualvalue) == "false":
return False
except:
pass
try:
actualvalue = json.loads(actualvalue)
except:
try:
actualvalue = int(str(actualvalue))
except:
try:
actualvalue = float(actualvalue)
except:
actualvalue = str(actualvalue)
return actualvalue
def populate_class_from_dict(myclass, options):
"""
:brief: helper function to establish a compatibility
layer between new way of storing config and current
way of accessing it. assumes all class attributes are
all-uppercase, and pattern-matches attributes in
priority order dict/list (json) -> -> int -> float-> string
REMEMBER TO ENCLOSE PROPERTY NAMES IN LISTS IN DOUBLE QUOTES
"""
for key, value in options:
if key.startswith("_") and key.endswith("options"):
continue
actualkey = key.upper()
actualvalue = conf_attribute_reader(value)
setattr(myclass, actualkey, actualvalue)
class TriggerMode:
SOFTWARE = "Software Trigger"
HARDWARE = "Hardware Trigger"
CONTINUOUS = "Continuous Acquisition"
class Acquisition:
NUMBER_OF_FOVS_PER_AF = 3
IMAGE_FORMAT = "bmp"
IMAGE_DISPLAY_SCALING_FACTOR = 0.3
DX = 0.9
DY = 0.9
DZ = 1.5
NX = 1
NY = 1
class PosUpdate:
INTERVAL_MS = 25
class MicrocontrollerDef:
MSG_LENGTH = 24
CMD_LENGTH = 24
N_BYTES_POS = 4
USE_SEPARATE_MCU_FOR_DAC = False
class MCU_PINS:
PWM1 = 5
PWM2 = 4
PWM3 = 22
PWM4 = 3
PWM5 = 23
PWM6 = 2
PWM7 = 1
PWM9 = 6
PWM10 = 7
PWM11 = 8
PWM12 = 9
PWM13 = 10
PWM14 = 15
PWM15 = 24
PWM16 = 25
AF_LASER = 15
class CMD_SET:
MOVE_X = 0
MOVE_Y = 1
MOVE_Z = 2
MOVE_THETA = 3
MOVE_W = 4
HOME_OR_ZERO = 5
TURN_ON_ILLUMINATION = 10
TURN_OFF_ILLUMINATION = 11
SET_ILLUMINATION = 12
SET_ILLUMINATION_LED_MATRIX = 13
ACK_JOYSTICK_BUTTON_PRESSED = 14
ANALOG_WRITE_ONBOARD_DAC = 15
SET_DAC80508_REFDIV_GAIN = 16
SET_ILLUMINATION_INTENSITY_FACTOR = 17
MOVETO_W = 18
MOVETO_X = 6
MOVETO_Y = 7
MOVETO_Z = 8
SET_LIM = 9
SET_LIM_SWITCH_POLARITY = 20
CONFIGURE_STEPPER_DRIVER = 21
SET_MAX_VELOCITY_ACCELERATION = 22
SET_LEAD_SCREW_PITCH = 23
SET_OFFSET_VELOCITY = 24
CONFIGURE_STAGE_PID = 25
ENABLE_STAGE_PID = 26
DISABLE_STAGE_PID = 27
SET_HOME_SAFETY_MERGIN = 28
SET_PID_ARGUMENTS = 29
SEND_HARDWARE_TRIGGER = 30
SET_STROBE_DELAY = 31
SET_AXIS_DISABLE_ENABLE = 32
MOVE_XY = 33
MOVETO_XY = 34
SET_SRAMP_MOD = 36
SET_PIN_LEVEL = 41
INITFILTERWHEEL = 253
INITIALIZE = 254
RESET = 255
class CMD_SET2:
ANALOG_WRITE_DAC8050X = 0
SET_CAMERA_TRIGGER_FREQUENCY = 1
START_CAMERA_TRIGGERING = 2
STOP_CAMERA_TRIGGERING = 3
BIT_POS_JOYSTICK_BUTTON = 0
BIT_POS_SWITCH = 1
class HOME_OR_ZERO:
HOME_NEGATIVE = 1 # motor moves along the negative direction (MCU coordinates)
HOME_POSITIVE = 0 # motor moves along the negative direction (MCU coordinates)
ZERO = 2
class AXIS:
X = 0
Y = 1
Z = 2
THETA = 3
XY = 4
W = 5
class LIMIT_CODE:
X_POSITIVE = 0
X_NEGATIVE = 1
Y_POSITIVE = 2
Y_NEGATIVE = 3
Z_POSITIVE = 4
Z_NEGATIVE = 5
class LIMIT_SWITCH_POLARITY:
ACTIVE_LOW = 0
ACTIVE_HIGH = 1
DISABLED = 2
X_HOME = 1
Y_HOME = 1
Z_HOME = 2
class ILLUMINATION_CODE:
ILLUMINATION_SOURCE_LED_ARRAY_FULL = 0
ILLUMINATION_SOURCE_LED_ARRAY_LEFT_HALF = 1
ILLUMINATION_SOURCE_LED_ARRAY_RIGHT_HALF = 2
ILLUMINATION_SOURCE_LED_ARRAY_LEFTB_RIGHTR = 3
ILLUMINATION_SOURCE_LED_ARRAY_LOW_NA = 4
ILLUMINATION_SOURCE_LED_ARRAY_LEFT_DOT = 5
ILLUMINATION_SOURCE_LED_ARRAY_RIGHT_DOT = 6
ILLUMINATION_SOURCE_LED_EXTERNAL_FET = 20
ILLUMINATION_SOURCE_405NM = 11
ILLUMINATION_SOURCE_488NM = 12
ILLUMINATION_SOURCE_638NM = 13
ILLUMINATION_SOURCE_561NM = 14
ILLUMINATION_SOURCE_730NM = 15
class VOLUMETRIC_IMAGING:
NUM_PLANES_PER_VOLUME = 20
class CMD_EXECUTION_STATUS:
COMPLETED_WITHOUT_ERRORS = 0
IN_PROGRESS = 1
CMD_CHECKSUM_ERROR = 2
CMD_INVALID = 3
CMD_EXECUTION_ERROR = 4
ERROR_CODE_EMPTYING_THE_FLUDIIC_LINE_FAILED = 100
class SpotDetectionMode(Enum):
"""Specifies which spot to detect when multiple spots are present.
SINGLE: Expect and detect single spot
DUAL_RIGHT: In dual-spot case, use rightmost spot
DUAL_LEFT: In dual-spot case, use leftmost spot
MULTI_RIGHT: In multi-spot case, use rightmost spot
MULTI_SECOND_RIGHT: In multi-spot case, use spot immediately left of rightmost spot
"""
SINGLE = "single"
DUAL_RIGHT = "dual_right"
DUAL_LEFT = "dual_left"
MULTI_RIGHT = "multi_right"
MULTI_SECOND_RIGHT = "multi_second_right"
class FileSavingOption(Enum):
"""File saving options.
INDIVIDUAL_IMAGES: Save each image as a separate file. Format is defined in Acquisition.IMAGE_FORMAT.
TODO: Move all file saving related settings to this enum.
MULTI_PAGE_TIFF: Save all images from a single FOV as a single multi-page TIFF file.
TODO: Add zarr saving options.
"""
INDIVIDUAL_IMAGES = "INDIVIDUAL_IMAGES"
MULTI_PAGE_TIFF = "MULTI_PAGE_TIFF"
@staticmethod
def convert_to_enum(option: Union[str, "FileSavingOption"]) -> "FileSavingOption":
"""
Attempts to convert the given string to a FileSavingOption. This ignores all letter cases.
"""
if isinstance(option, FileSavingOption):
return option
try:
return FileSavingOption[option.upper()]
except KeyError:
raise ValueError(f"Invalid file saving option: {option}")
class FocusMeasureOperator(Enum):
LAPE = "LAPE" # LAPE has worked well for bright field images
GLVA = "GLVA" # GLVA works well for darkfield/fluorescence
TENENGRAD = "TENENGRAD"
@staticmethod
def convert_to_enum(option: Union[str, "FocusMeasureOperator"]) -> "FocusMeasureOperator":
"""
Attempts to convert the given string to a FocusMeasureOperator. This ignores all letter cases.
"""
if isinstance(option, FocusMeasureOperator):
return option
try:
return FocusMeasureOperator[option.upper()]
except KeyError:
raise ValueError(f"Invalid focus measure operator: {option}")
PRINT_CAMERA_FPS = True
###########################################################
#### machine specific configurations - to be overridden ###
###########################################################
DEFAULT_TRIGGER_MODE = TriggerMode.SOFTWARE
BUFFER_SIZE_LIMIT = 4095
# note: XY are the in-plane axes, Z is the focus axis
# change the following so that "backward" is "backward" - towards the single sided hall effect sensor
STAGE_MOVEMENT_SIGN_X = -1
STAGE_MOVEMENT_SIGN_Y = 1
STAGE_MOVEMENT_SIGN_Z = -1
STAGE_MOVEMENT_SIGN_THETA = 1
STAGE_MOVEMENT_SIGN_W = 1
STAGE_POS_SIGN_X = STAGE_MOVEMENT_SIGN_X
STAGE_POS_SIGN_Y = STAGE_MOVEMENT_SIGN_Y
STAGE_POS_SIGN_Z = STAGE_MOVEMENT_SIGN_Z
STAGE_POS_SIGN_THETA = STAGE_MOVEMENT_SIGN_THETA
TRACKING_MOVEMENT_SIGN_X = 1
TRACKING_MOVEMENT_SIGN_Y = 1
TRACKING_MOVEMENT_SIGN_Z = 1
TRACKING_MOVEMENT_SIGN_THETA = 1
USE_ENCODER_X = False
USE_ENCODER_Y = False
USE_ENCODER_Z = False
USE_ENCODER_THETA = False
ENCODER_POS_SIGN_X = 1
ENCODER_POS_SIGN_Y = 1
ENCODER_POS_SIGN_Z = 1
ENCODER_POS_SIGN_THETA = 1
ENCODER_STEP_SIZE_X_MM = 100e-6
ENCODER_STEP_SIZE_Y_MM = 100e-6
ENCODER_STEP_SIZE_Z_MM = 100e-6
ENCODER_STEP_SIZE_THETA = 1
FULLSTEPS_PER_REV_X = 200
FULLSTEPS_PER_REV_Y = 200
FULLSTEPS_PER_REV_Z = 200
FULLSTEPS_PER_REV_W = 200
FULLSTEPS_PER_REV_THETA = 200
# beginning of actuator specific configurations
SCREW_PITCH_X_MM = 1
SCREW_PITCH_Y_MM = 1
SCREW_PITCH_Z_MM = 0.012 * 25.4
SCREW_PITCH_W_MM = 1
MICROSTEPPING_DEFAULT_X = 8
MICROSTEPPING_DEFAULT_Y = 8
MICROSTEPPING_DEFAULT_Z = 8
MICROSTEPPING_DEFAULT_W = 64
MICROSTEPPING_DEFAULT_THETA = 8 # not used, to be removed
X_MOTOR_RMS_CURRENT_mA = 490
Y_MOTOR_RMS_CURRENT_mA = 490
Z_MOTOR_RMS_CURRENT_mA = 490
W_MOTOR_RMS_CURRENT_mA = 1900
X_MOTOR_I_HOLD = 0.5
Y_MOTOR_I_HOLD = 0.5
Z_MOTOR_I_HOLD = 0.5
W_MOTOR_I_HOLD = 0.5
MAX_VELOCITY_X_mm = 25
MAX_VELOCITY_Y_mm = 25
MAX_VELOCITY_Z_mm = 2
MAX_VELOCITY_W_mm = 3.19
MAX_ACCELERATION_X_mm = 500
MAX_ACCELERATION_Y_mm = 500
MAX_ACCELERATION_Z_mm = 20
MAX_ACCELERATION_W_mm = 300
# config encoder arguments
HAS_ENCODER_X = False
HAS_ENCODER_Y = False
HAS_ENCODER_Z = False
HAS_ENCODER_W = False
# enable PID control
ENABLE_PID_X = False
ENABLE_PID_Y = False
ENABLE_PID_Z = False
ENABLE_PID_W = False
# PID arguments
PID_P_X = int(1 << 12)
PID_I_X = int(0)
PID_D_X = int(0)
PID_P_Y = int(1 << 12)
PID_I_Y = int(0)
PID_D_Y = int(0)
PID_P_Z = int(1 << 12)
PID_I_Z = int(0)
PID_D_Z = int(1)
PID_P_W = int(1 << 12)
PID_I_W = int(1)
PID_D_W = int(1)
# flip direction True or False
ENCODER_FLIP_DIR_X = True
ENCODER_FLIP_DIR_Y = True
ENCODER_FLIP_DIR_Z = True
ENCODER_FLIP_DIR_W = False
# distance for each count (um)
ENCODER_RESOLUTION_UM_X = 0.05
ENCODER_RESOLUTION_UM_Y = 0.05
ENCODER_RESOLUTION_UM_Z = 0.1
# end of actuator specific configurations
SCAN_STABILIZATION_TIME_MS_X = 160
SCAN_STABILIZATION_TIME_MS_Y = 160
SCAN_STABILIZATION_TIME_MS_Z = 20
HOMING_ENABLED_X = True
HOMING_ENABLED_Y = True
HOMING_ENABLED_Z = False
SLEEP_TIME_S = 0.005
LED_MATRIX_R_FACTOR = 0
LED_MATRIX_G_FACTOR = 0
LED_MATRIX_B_FACTOR = 1
DEFAULT_SAVING_PATH = str(Path.home()) + "/Downloads"
FILE_ID_PADDING = 0
# 0: RAMP_HOLD
# 1: RAMP_TRAPEZ
# 2: RAMP_SSHAPE
X_AXIS_SRAMP_MODE = 2
Y_AXIS_SRAMP_MODE = 2
Z_AXIS_SRAMP_MODE = 2
W_AXIS_SRAMP_MODE = 2
class PLATE_READER:
NUMBER_OF_ROWS = 8
NUMBER_OF_COLUMNS = 12
ROW_SPACING_MM = 9
COLUMN_SPACING_MM = 9
OFFSET_COLUMN_1_MM = 20
OFFSET_ROW_A_MM = 20
CAMERA_PIXEL_SIZE_UM = {
"IMX290": 2.9,
"IMX178": 2.4,
"IMX226": 1.85,
"IMX250": 3.45,
"IMX252": 3.45,
"IMX273": 3.45,
"IMX264": 3.45,
"IMX265": 3.45,
"IMX571": 3.76,
"PYTHON300": 4.8,
}
TUBE_LENS_MM = 50
CAMERA_SENSOR = "IMX226"
TRACKERS = ["csrt", "kcf", "mil", "tld", "medianflow", "mosse", "daSiamRPN"]
DEFAULT_TRACKER = "csrt"
ENABLE_TRACKING = False
TRACKING_SHOW_MICROSCOPE_CONFIGURATIONS = False # set to true when doing multimodal acquisition
class CAMERA_CONFIG:
ROI_OFFSET_X_DEFAULT = None
ROI_OFFSET_Y_DEFAULT = None
ROI_WIDTH_DEFAULT = None
ROI_HEIGHT_DEFAULT = None
ROTATE_IMAGE_ANGLE = None
FLIP_IMAGE = None # 'Horizontal', 'Vertical', 'Both'
CROP_WIDTH_UNBINNED = 4168
CROP_HEIGHT_UNBINNED = 4168
BINNING_FACTOR_DEFAULT = 2
PIXEL_FORMAT_DEFAULT = "MONO12"
TEMPERATURE_DEFAULT = 20
FAN_SPEED_DEFAULT = 1
BLACKLEVEL_VALUE_DEFAULT = 3
AWB_RATIOS_R = 1.375
AWB_RATIOS_G = 1
AWB_RATIOS_B = 1.4141
class AF:
STOP_THRESHOLD = 0.85
CROP_WIDTH = 800
CROP_HEIGHT = 800
class Tracking:
SEARCH_AREA_RATIO = 10 # @@@ check
CROPPED_IMG_RATIO = 10 # @@@ check
BBOX_SCALE_FACTOR = 1.2
DEFAULT_TRACKER = "csrt"
INIT_METHODS = ["roi"]
DEFAULT_INIT_METHOD = "roi"
SHOW_DAC_CONTROL = False
class SLIDE_POSITION:
LOADING_X_MM = 30
LOADING_Y_MM = 55
SCANNING_X_MM = 3
SCANNING_Y_MM = 3
class OUTPUT_GAINS:
REFDIV = False
CHANNEL0_GAIN = False
CHANNEL1_GAIN = False
CHANNEL2_GAIN = False
CHANNEL3_GAIN = False
CHANNEL4_GAIN = False
CHANNEL5_GAIN = False
CHANNEL6_GAIN = False
CHANNEL7_GAIN = True
SLIDE_POTISION_SWITCHING_TIMEOUT_LIMIT_S = 10
SLIDE_POTISION_SWITCHING_HOME_EVERYTIME = False
class SOFTWARE_POS_LIMIT:
X_POSITIVE = 56
X_NEGATIVE = -0.5
Y_POSITIVE = 56
Y_NEGATIVE = -0.5
Z_POSITIVE = 7
Z_NEGATIVE = 0.05
SHOW_AUTOLEVEL_BTN = False
AUTOLEVEL_DEFAULT_SETTING = False
MULTIPOINT_AUTOFOCUS_CHANNEL = "BF LED matrix full"
# MULTIPOINT_AUTOFOCUS_CHANNEL = 'BF LED matrix left half'
MULTIPOINT_AUTOFOCUS_ENABLE_BY_DEFAULT = False
MULTIPOINT_BF_SAVING_OPTION = "Raw"
# MULTIPOINT_BF_SAVING_OPTION = 'RGB2GRAY'
# MULTIPOINT_BF_SAVING_OPTION = 'Green Channel Only'
DEFAULT_MULTIPOINT_NX = 1
DEFAULT_MULTIPOINT_NY = 1
ENABLE_FLEXIBLE_MULTIPOINT = True
USE_OVERLAP_FOR_FLEXIBLE = True
ENABLE_WELLPLATE_MULTIPOINT = True
ENABLE_RECORDING = False
RESUME_LIVE_AFTER_ACQUISITION = True
CAMERA_SN = {"ch 1": "SN1", "ch 2": "SN2"} # for multiple cameras, to be overwritten in the configuration file
ENABLE_STROBE_OUTPUT = False
ACQUISITION_PATTERN = "S-Pattern" # 'S-Pattern', 'Unidirectional'
FOV_PATTERN = "Unidirectional" # 'S-Pattern', 'Unidirectional'
Z_STACKING_CONFIG = "FROM BOTTOM" # 'FROM BOTTOM', 'FROM TOP'
Z_STACKING_CONFIG_MAP = {0: "FROM BOTTOM", 1: "FROM CENTER", 2: "FROM TOP"}
DEFAULT_Z_POS_MM = 2
WELLPLATE_OFFSET_X_mm = 0 # x offset adjustment for using different plates
WELLPLATE_OFFSET_Y_mm = 0 # y offset adjustment for using different plates
# for USB spectrometer
N_SPECTRUM_PER_POINT = 5
# focus measure operator
FOCUS_MEASURE_OPERATOR = FocusMeasureOperator.LAPE
# controller version
CONTROLLER_VERSION = "Arduino Due" # 'Teensy'
# How to read Spinnaker nodemaps, options are INDIVIDUAL or VALUE
CHOSEN_READ = "INDIVIDUAL"
# laser autofocus
SUPPORT_LASER_AUTOFOCUS = True
MAIN_CAMERA_MODEL = "MER2-1220-32U3M"
FOCUS_CAMERA_MODEL = "MER2-630-60U3M"
FOCUS_CAMERA_EXPOSURE_TIME_MS = 2
FOCUS_CAMERA_ANALOG_GAIN = 0
LASER_AF_AVERAGING_N = 3
LASER_AF_DISPLAY_SPOT_IMAGE = True
LASER_AF_CROP_WIDTH = 1536
LASER_AF_CROP_HEIGHT = 256
LASER_AF_SPOT_DETECTION_MODE = SpotDetectionMode.DUAL_LEFT.value
LASER_AF_RANGE = 100
DISPLACEMENT_SUCCESS_WINDOW_UM = 1.0
SPOT_CROP_SIZE = 100
CORRELATION_THRESHOLD = 0.9
PIXEL_TO_UM_CALIBRATION_DISTANCE = 6.0
LASER_AF_Y_WINDOW = 96
LASER_AF_X_WINDOW = 20
LASER_AF_MIN_PEAK_WIDTH = 10
LASER_AF_MIN_PEAK_DISTANCE = 10
LASER_AF_MIN_PEAK_PROMINENCE = 0.25
LASER_AF_SPOT_SPACING = 100
SHOW_LEGACY_DISPLACEMENT_MEASUREMENT_WINDOWS = False
MULTIPOINT_REFLECTION_AUTOFOCUS_ENABLE_BY_DEFAULT = False
MULTIPOINT_CONTRAST_AUTOFOCUS_ENABLE_BY_DEFAULT = False
RUN_CUSTOM_MULTIPOINT = False
RETRACT_OBJECTIVE_BEFORE_MOVING_TO_LOADING_POSITION = True
OBJECTIVE_RETRACTED_POS_MM = 0.1
TWO_CLASSIFICATION_MODELS = False
CLASSIFICATION_MODEL_PATH = "models/resnet18_en/version1/best.pt"
CLASSIFICATION_MODEL_PATH2 = "models/resnet18_en/version2/best.pt"
CLASSIFICATION_TEST_MODE = False
CLASSIFICATION_TH = 0.3
SEGMENTATION_MODEL_PATH = "models/m2unet_model_flat_erode1_wdecay5_smallbatch/model_4000_11.pth"
ENABLE_SEGMENTATION = True
USE_TRT_SEGMENTATION = False
SEGMENTATION_CROP = 1500
DISP_TH_DURING_MULTIPOINT = 0.95
SORT_DURING_MULTIPOINT = False
INVERTED_OBJECTIVE = False
ILLUMINATION_INTENSITY_FACTOR = 0.6
CAMERA_TYPE = "Default"
FOCUS_CAMERA_TYPE = "Default"
# Spinning disk confocal integration
ENABLE_SPINNING_DISK_CONFOCAL = False
USE_LDI_SERIAL_CONTROL = False
LDI_INTENSITY_MODE = "PC"
LDI_SHUTTER_MODE = "PC"
USE_CELESTA_ETHENET_CONTROL = False
XLIGHT_EMISSION_FILTER_MAPPING = {
405: 1,
470: 1,
555: 1,
640: 1,
730: 1,
} # TODO: This is not being used. Need to map wavelength to illumination source in LiveController
XLIGHT_SERIAL_NUMBER = "B00031BE"
XLIGHT_SLEEP_TIME_FOR_WHEEL = 0.25
XLIGHT_VALIDATE_WHEEL_POS = False
XLIGHT_ILLUMINATION_IRIS_DEFAULT = 100
XLIGHT_EMISSION_IRIS_DEFAULT = 100
# Confocal.nl NL5 integration
ENABLE_NL5 = False
ENABLE_CELLX = False
CELLX_SN = None
CELLX_MODULATION = "EXT Digital"
NL5_USE_AOUT = False
NL5_USE_DOUT = True
NL5_TRIGGER_PIN = 2
NL5_WAVENLENGTH_MAP = {405: 1, 470: 2, 488: 2, 545: 3, 555: 3, 561: 3, 637: 4, 638: 4, 640: 4}
# Laser AF characterization mode
LASER_AF_CHARACTERIZATION_MODE = False
# Napari integration
USE_NAPARI_FOR_LIVE_VIEW = False
USE_NAPARI_FOR_MULTIPOINT = True
USE_NAPARI_FOR_MOSAIC_DISPLAY = True
USE_NAPARI_WELL_SELECTION = False
USE_NAPARI_FOR_LIVE_CONTROL = False
LIVE_ONLY_MODE = False
MOSAIC_VIEW_TARGET_PIXEL_SIZE_UM = 2
# Controller SN (needed when using multiple teensy-based connections)
CONTROLLER_SN = None
# Sci microscopy
SUPPORT_SCIMICROSCOPY_LED_ARRAY = False
SCIMICROSCOPY_LED_ARRAY_SN = None
SCIMICROSCOPY_LED_ARRAY_DISTANCE = 50
SCIMICROSCOPY_LED_ARRAY_DEFAULT_NA = 0.8
SCIMICROSCOPY_LED_ARRAY_DEFAULT_COLOR = [1, 1, 1]
SCIMICROSCOPY_LED_ARRAY_TURN_ON_DELAY = 0.03 # time to wait before trigger the camera (in seconds)
# Navigation Settings
ENABLE_CLICK_TO_MOVE_BY_DEFAULT = True
# Stitcher
ENABLE_STITCHER = False
IS_HCS = False
DYNAMIC_REGISTRATION = False
STITCH_COMPLETE_ACQUISITION = False
# Pseudo color settings
CHANNEL_COLORS_MAP = {
"405": {"hex": 0x20ADF8, "name": "bop blue"},
"488": {"hex": 0x1FFF00, "name": "green"},
"561": {"hex": 0xFFCF00, "name": "yellow"},
"638": {"hex": 0xFF0000, "name": "red"},
"730": {"hex": 0x770000, "name": "dark red"},
"R": {"hex": 0xFF0000, "name": "red"},
"G": {"hex": 0x1FFF00, "name": "green"},
"B": {"hex": 0x3300FF, "name": "blue"},
}
SAVE_IN_PSEUDO_COLOR = False
MERGE_CHANNELS = False
# Emission filter wheel
USE_ZABER_EMISSION_FILTER_WHEEL = False
ZABER_EMISSION_FILTER_WHEEL_DELAY_MS = 70
ZABER_EMISSION_FILTER_WHEEL_BLOCKING_CALL = False
USE_OPTOSPIN_EMISSION_FILTER_WHEEL = False
FILTER_CONTROLLER_SERIAL_NUMBER = "A10NG007"
OPTOSPIN_EMISSION_FILTER_WHEEL_SPEED_HZ = 50
OPTOSPIN_EMISSION_FILTER_WHEEL_DELAY_MS = 70
OPTOSPIN_EMISSION_FILTER_WHEEL_TTL_TRIGGER = False
USE_SQUID_FILTERWHEEL = False
SQUID_FILTERWHEEL_MAX_INDEX = 8
SQUID_FILTERWHEEL_MIN_INDEX = 1
SQUID_FILTERWHEEL_OFFSET = 0.008
SQUID_FILTERWHEEL_HOMING_ENABLED = True
SQUID_FILTERWHEEL_MOTORSLOTINDEX = 3
SQUID_FILTERWHEEL_TRANSITIONS_PER_REVOLUTION = 4000
# Stage
USE_PRIOR_STAGE = False
PRIOR_STAGE_SN = ""
# camera blacklevel settings
DISPLAY_TOUPCAMER_BLACKLEVEL_SETTINGS = False
def read_objectives_csv(file_path):
objectives = {}
with open(file_path, "r") as csvfile:
reader = csv.DictReader(csvfile)
for row in reader:
objectives[row["name"]] = {
"magnification": float(row["magnification"]),
"NA": float(row["NA"]),
"tube_lens_f_mm": float(row["tube_lens_f_mm"]),
}
return objectives
def read_sample_formats_csv(file_path):
sample_formats = {}
with open(file_path, "r") as csvfile:
reader = csv.DictReader(csvfile)
for row in reader:
format_ = str(row["format"])
format_key = f"{format_} well plate" if format_.isdigit() else format_
sample_formats[format_key] = {
"a1_x_mm": float(row["a1_x_mm"]),
"a1_y_mm": float(row["a1_y_mm"]),
"a1_x_pixel": int(row["a1_x_pixel"]),
"a1_y_pixel": int(row["a1_y_pixel"]),
"well_size_mm": float(row["well_size_mm"]),
"well_spacing_mm": float(row["well_spacing_mm"]),
"number_of_skip": int(row["number_of_skip"]),
"rows": int(row["rows"]),
"cols": int(row["cols"]),
}
return sample_formats
def load_formats():
"""Load formats, prioritizing cache for sample formats."""
cache_path = "cache"
default_path = "objective_and_sample_formats"
# Load objectives (from default location)
objectives = read_objectives_csv(os.path.join(default_path, "objectives.csv"))
# Try cache first for sample formats, fall back to default if not found
cached_formats_path = os.path.join(cache_path, "sample_formats.csv")
default_formats_path = os.path.join(default_path, "sample_formats.csv")
if os.path.exists(cached_formats_path):
print("Using cached sample formats")
sample_formats = read_sample_formats_csv(cached_formats_path)
else:
print("Using default sample formats")
sample_formats = read_sample_formats_csv(default_formats_path)
return objectives, sample_formats
OBJECTIVES_CSV_PATH = "objectives.csv"
SAMPLE_FORMATS_CSV_PATH = "sample_formats.csv"
OBJECTIVES, WELLPLATE_FORMAT_SETTINGS = load_formats()
# limit switch
X_HOME_SWITCH_POLARITY = LIMIT_SWITCH_POLARITY.X_HOME
Y_HOME_SWITCH_POLARITY = LIMIT_SWITCH_POLARITY.Y_HOME
Z_HOME_SWITCH_POLARITY = LIMIT_SWITCH_POLARITY.Z_HOME
# home safety margin with (um) unit
X_HOME_SAFETY_MARGIN_UM = 50
Y_HOME_SAFETY_MARGIN_UM = 50
Z_HOME_SAFETY_MARGIN_UM = 600
USE_XERYON = False
XERYON_SERIAL_NUMBER = "95130303033351E02050"
XERYON_SPEED = 80
XERYON_OBJECTIVE_SWITCHER_POS_1 = ["4x", "10x"]
XERYON_OBJECTIVE_SWITCHER_POS_2 = ["20x", "40x", "60x"]
XERYON_OBJECTIVE_SWITCHER_POS_2_OFFSET_MM = 2
# fluidics
RUN_FLUIDICS = False
FLUIDICS_CONFIG_PATH = "./merfish_config/MERFISH_config.json"
USE_TEMPLATE_MULTIPOINT = False
FILE_SAVING_OPTION = FileSavingOption.INDIVIDUAL_IMAGES
##########################################################
#### start of loading machine specific configurations ####
##########################################################
CACHED_CONFIG_FILE_PATH = None
# Piezo configuration items
Z_MOTOR_CONFIG = "STEPPER" # "STEPPER", "STEPPER + PIEZO", "PIEZO", "LINEAR"
# the value of OBJECTIVE_PIEZO_CONTROL_VOLTAGE_RANGE is 2.5 or 5
OBJECTIVE_PIEZO_CONTROL_VOLTAGE_RANGE = 5
OBJECTIVE_PIEZO_RANGE_UM = 300
OBJECTIVE_PIEZO_HOME_UM = 20
OBJECTIVE_PIEZO_FLIP_DIR = False
MULTIPOINT_PIEZO_DELAY_MS = 20
MULTIPOINT_PIEZO_UPDATE_DISPLAY = True
USE_TERMINAL_CONSOLE = False
USE_JUPYTER_CONSOLE = False
try:
with open("cache/config_file_path.txt", "r") as file:
for line in file:
CACHED_CONFIG_FILE_PATH = line
break
except FileNotFoundError:
CACHED_CONFIG_FILE_PATH = None
config_files = glob.glob("." + "/" + "configuration*.ini")
if config_files:
if len(config_files) > 1:
if CACHED_CONFIG_FILE_PATH in config_files:
log.info(f"defaulting to last cached config file at '{CACHED_CONFIG_FILE_PATH}'")
config_files = [CACHED_CONFIG_FILE_PATH]
else:
log.error("multiple machine configuration files found, the program will exit")
sys.exit(1)
log.info("load machine-specific configuration")
# exec(open(config_files[0]).read())
cfp = ConfigParser()
cfp.read(config_files[0])
var_items = list(locals().keys())
for var_name in var_items:
if type(locals()[var_name]) is type:
continue
varnamelower = var_name.lower()
if varnamelower not in cfp.options("GENERAL"):
continue
value = cfp.get("GENERAL", varnamelower)
actualvalue = conf_attribute_reader(value)
locals()[var_name] = actualvalue
for classkey in var_items:
myclass = None
classkeyupper = classkey.upper()
pop_items = None
try:
pop_items = cfp.items(classkeyupper)
except:
continue
if type(locals()[classkey]) is not type:
continue
myclass = locals()[classkey]
populate_class_from_dict(myclass, pop_items)
with open("cache/config_file_path.txt", "w") as file:
file.write(config_files[0])
CACHED_CONFIG_FILE_PATH = config_files[0]
else:
log.warning("configuration*.ini file not found, defaulting to legacy configuration")
config_files = glob.glob("." + "/" + "configuration*.txt")
if config_files:
if len(config_files) > 1:
log.error("multiple machine configuration files found, the program will exit")
sys.exit(1)
log.info("load machine-specific configuration")
exec(open(config_files[0]).read())
else:
log.error("machine-specific configuration not present, the program will exit")
sys.exit(1)
try:
with open("cache/objective_and_sample_format.txt", "r") as f:
cached_settings = json.load(f)
DEFAULT_OBJECTIVE = (
cached_settings.get("objective") if cached_settings.get("objective") in OBJECTIVES else "20x"
)
WELLPLATE_FORMAT = str(cached_settings.get("wellplate_format"))
WELLPLATE_FORMAT = WELLPLATE_FORMAT + " well plate" if WELLPLATE_FORMAT.isdigit() else WELLPLATE_FORMAT
if WELLPLATE_FORMAT not in WELLPLATE_FORMAT_SETTINGS:
WELLPLATE_FORMAT = "96 well plate"
except (FileNotFoundError, json.JSONDecodeError):
DEFAULT_OBJECTIVE = "20x"
WELLPLATE_FORMAT = "96 well plate"
NUMBER_OF_SKIP = WELLPLATE_FORMAT_SETTINGS[WELLPLATE_FORMAT][
"number_of_skip"
] # num rows/cols to skip on wellplate edge
WELL_SIZE_MM = WELLPLATE_FORMAT_SETTINGS[WELLPLATE_FORMAT]["well_size_mm"]
WELL_SPACING_MM = WELLPLATE_FORMAT_SETTINGS[WELLPLATE_FORMAT]["well_spacing_mm"]
A1_X_MM = WELLPLATE_FORMAT_SETTINGS[WELLPLATE_FORMAT]["a1_x_mm"] # measured stage position - to update
A1_Y_MM = WELLPLATE_FORMAT_SETTINGS[WELLPLATE_FORMAT]["a1_y_mm"] # measured stage position - to update
A1_X_PIXEL = WELLPLATE_FORMAT_SETTINGS[WELLPLATE_FORMAT]["a1_x_pixel"] # coordinate on the png
A1_Y_PIXEL = WELLPLATE_FORMAT_SETTINGS[WELLPLATE_FORMAT]["a1_y_pixel"] # coordinate on the png
##########################################################
##### end of loading machine specific configurations #####
##########################################################
# objective piezo
HAS_OBJECTIVE_PIEZO = "PIEZO" in Z_MOTOR_CONFIG
MULTIPOINT_USE_PIEZO_FOR_ZSTACKS = HAS_OBJECTIVE_PIEZO
# convert str to enum
FILE_SAVING_OPTION = FileSavingOption.convert_to_enum(FILE_SAVING_OPTION)
FOCUS_MEASURE_OPERATOR = FocusMeasureOperator.convert_to_enum(FOCUS_MEASURE_OPERATOR)
# saving path
if not (DEFAULT_SAVING_PATH.startswith(str(Path.home()))):
DEFAULT_SAVING_PATH = str(Path.home()) + "/" + DEFAULT_SAVING_PATH.strip("/")