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This repository was archived by the owner on Apr 20, 2026. It is now read-only.
This repository was archived by the owner on Apr 20, 2026. It is now read-only.

Can I disable the SB flag when not used? #75

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@Manuel-DominguezCBG

In our test, we only obtained R1 reads. I was wondering if there is a way to not flag variants based on SB. Is this possible?

My code

apptainer exec --bind "$ref_folder":"$ref_folder" \
               "$pisces"  dotnet /app/Pisces_5.2.9.122/Pisces.dll \
               -bam  "${Sample_ID}"_FINAL_SORTED.bam \
               -g  "$piscesrefgenomefolder" \
               -o ./ \
               -i "$ampliconscoordenatesbed"  \
               --gvcf false \
               --minvq 20 \
               --maxvq 100 \
               --minbq 20 \
               --mindpfilter 5000 \
               --ssfilter False\
               --minmq 20 \
               --mindp 10 

and a couple of variants


#CHROM	POS	ID	REF	ALT	QUAL	FILTER	INFO	FORMAT	2111700067_FINAL_SORTED.bam
chr5	171410472	.	G	C	80	LowDP;NC;SB	DP=14	GT:GQ:AD:DP:VF:NL:SB	0/1:80:4,6:14:0.429:20:-100.0000
chr5	171410482	.	A	T	100	LowDP;NC;SB	DP=969	GT:GQ:AD:DP:VF:NL:SB	0/1:100:919,49:969:0.051:20:-100.0000

I know that the tool is not filtering out variant based on SB but it is weird that the flag is still there.

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