Dear developers,
I'm creating datasets from multiple genomes at 20X coverage and then concatenating these genomes with "cat" to get .R1.fq and .R2.fq files, then using the spades assembler and further testing some other software with the assembled fasta file.
Does the location of reads in .fq files matter for SPADES' algorithm? Would you suggest randomizing the concentration of these multiple .fq files?
Thanks in advance.
Başak
Dear developers,
I'm creating datasets from multiple genomes at 20X coverage and then concatenating these genomes with "cat" to get .R1.fq and .R2.fq files, then using the spades assembler and further testing some other software with the assembled fasta file.
Does the location of reads in .fq files matter for SPADES' algorithm? Would you suggest randomizing the concentration of these multiple .fq files?
Thanks in advance.
Başak