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README.md

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# Processed Cell Painting Data for the LINCS Drug Repurposing Project
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# Processed Data for the LINCS Cell Painting Project
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The repository stores data and data processing scripts for **a subset** of the LINCS Drug Repurposing Project.
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In this project, we perturbed A549 cells with ~1,500 compounds across 6 doses with ~4 biological replicates.
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The repository stores data and data processing scripts for **a subset** of the [Broad Drug Repurposing Hub](https://clue.io/repurposing#home) collection of compounds.
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For a specific list of compounds tested, see [`metadata`](https://github.com/broadinstitute/lincs-cell-painting/tree/master/metadata).
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In this project, the [Connectivity Map](https://clue.io/team) team perturbed A549 cells with ~1,500 compounds across 6 doses in 5 technical replicates.
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We refer to this dataset as `LINCS Pilot 1`.
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For a specific list of compounds tested, see [`metadata`](https://github.com/broadinstitute/lincs-cell-painting/tree/master/metadata).
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Information about the compounds can be interactively explored in the [CLUE Repurposing app](https://clue.io/repurposing-app).
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The [Morphology Connectivity Hub](https://clue.io/morphology) is the primary source of this dataset.
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## Image-Based Profiling
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We apply a unified, image-based profiling pipeline to all Drug Repurposing Hub Cell Painting plates.
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We apply a unified, image-based profiling pipeline to all 136 384-well plates from `LINCS Pilot 1` .
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We use [pycytominer](https://github.com/cytomining/pycytominer) as the primary tool for image-based profiling.
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The profiles are processed and stored in the [profiles/](profiles/) directory.

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