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RNA-seq Processor.app/Contents/Resources/app Expand file tree Collapse file tree Original file line number Diff line number Diff line change @@ -1213,6 +1213,21 @@ def update_progress(msg):
12131213 est_minutes = 1
12141214 st .info (f"⏱️ Estimated processing time: **~{ est_minutes } minutes** for { n_samples } samples at { threads } threads" )
12151215
1216+ with st .expander ("View quantification command" ):
1217+ st .code (
1218+ f"salmon quant \\ \n "
1219+ f" --index <index_dir> \\ \n "
1220+ f" --libType A \\ \n "
1221+ f" --mates1 <R1.fastq.gz> \\ \n "
1222+ f" --mates2 <R2.fastq.gz> \\ \n "
1223+ f" --output <output_dir> \\ \n "
1224+ f" --threads { threads } \\ \n "
1225+ f" --validateMappings \\ \n "
1226+ f" --gcBias \\ \n "
1227+ f" --seqBias" ,
1228+ language = "bash"
1229+ )
1230+
12161231 # Run button
12171232 if st .button ("🚀 Start Quantification" , type = "primary" , disabled = st .session_state .pipeline_running ):
12181233 st .session_state .pipeline_running = True
@@ -1772,15 +1787,6 @@ def run_pipeline_thread():
17721787 3. Run analysis
17731788 """ )
17741789
1775- st .info (
1776- "**What's running under the hood:** Salmon (quasi-mapping) quantifies reads at the "
1777- "transcript level. tximport summarizes to gene-level counts using the "
1778- "`lengthScaledTPM` method. DESeq2 normalizes counts (median-of-ratios), applies "
1779- "variance stabilizing transformation (VST) for PCA, and runs Wald tests for "
1780- "differential expression. GSEA is performed with fgsea using pre-ranked gene lists "
1781- "(ranked by log2 fold change)."
1782- )
1783-
17841790 with st .expander ("View analysis code (analysis.R)" ):
17851791 r_script = Path (__file__ ).parent / "analysis.R"
17861792 if r_script .exists ():
Original file line number Diff line number Diff line change @@ -1285,6 +1285,21 @@ def update_progress(msg):
12851285 est_minutes = 1
12861286 st .info (f"⏱️ Estimated processing time: **~{ est_minutes } minutes** for { n_samples } samples at { threads } threads" )
12871287
1288+ with st .expander ("View quantification command" ):
1289+ st .code (
1290+ f"salmon quant \\ \n "
1291+ f" --index <index_dir> \\ \n "
1292+ f" --libType A \\ \n "
1293+ f" --mates1 <R1.fastq.gz> \\ \n "
1294+ f" --mates2 <R2.fastq.gz> \\ \n "
1295+ f" --output <output_dir> \\ \n "
1296+ f" --threads { threads } \\ \n "
1297+ f" --validateMappings \\ \n "
1298+ f" --gcBias \\ \n "
1299+ f" --seqBias" ,
1300+ language = "bash"
1301+ )
1302+
12881303 # Run button
12891304 if st .button ("🚀 Start Quantification" , type = "primary" , disabled = st .session_state .pipeline_running ):
12901305 st .session_state .pipeline_running = True
@@ -1875,15 +1890,6 @@ def run_pipeline_thread():
18751890 3. Run analysis
18761891 """ )
18771892
1878- st .info (
1879- "**What's running under the hood:** Salmon (quasi-mapping) quantifies reads at the "
1880- "transcript level. tximport summarizes to gene-level counts using the "
1881- "`lengthScaledTPM` method. DESeq2 normalizes counts (median-of-ratios), applies "
1882- "variance stabilizing transformation (VST) for PCA, and runs Wald tests for "
1883- "differential expression. GSEA is performed with fgsea using pre-ranked gene lists "
1884- "(ranked by log2 fold change)."
1885- )
1886-
18871893 with st .expander ("View analysis code (analysis.R)" ):
18881894 r_script = Path (__file__ ).parent / "analysis.R"
18891895 if r_script .exists ():
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