Skip to content

Commit 8ad5954

Browse files
committed
Rename Taxonomic database section in schema to Taxnomic assignment, and move dada_min_boot there
1 parent ae720bc commit 8ad5954

3 files changed

Lines changed: 9 additions & 23 deletions

File tree

docs/usage.md

Lines changed: 1 addition & 1 deletion
Original file line numberDiff line numberDiff line change
@@ -250,7 +250,7 @@ Special features of taxonomic classification tools:
250250
- QIIME2's reference taxonomy databases will have regions matching the amplicon extracted with primer sequences.
251251
- DADA2, Kraken2, and QIIME2 have specific parameters to accept custom databases (but theoretically possible with all classifiers)
252252

253-
Parameter guidance is given in [nf-core/ampliseq website parameter documentation](https://nf-co.re/ampliseq/parameters/#taxonomic-database). Citations are listed in [`CITATIONS.md`](CITATIONS.md).
253+
Parameter guidance is given in [nf-core/ampliseq website parameter documentation](https://nf-co.re/ampliseq/parameters/#taxonomic-assignment). Citations are listed in [`CITATIONS.md`](CITATIONS.md).
254254

255255
### Multiple region analysis with Sidle
256256

nextflow.config

Lines changed: 1 addition & 3 deletions
Original file line numberDiff line numberDiff line change
@@ -119,10 +119,8 @@ params {
119119
skip_multiqc = false
120120
skip_report = false
121121

122-
// DADA2 assignTaxonomy options
122+
// Taxonomic assignment options
123123
dada_min_boot = 50
124-
125-
// Database options
126124
dada_ref_taxonomy = "silva=138.2"
127125
dada_assign_taxlevels = null
128126
dada_ref_tax_custom = null

nextflow_schema.json

Lines changed: 7 additions & 19 deletions
Original file line numberDiff line numberDiff line change
@@ -405,29 +405,20 @@
405405
},
406406
"fa_icon": "fas fa-filter"
407407
},
408-
"dada2_assigntaxonomy": {
409-
"title": "DADA2 assignTaxonomy",
408+
"taxonomic_assignment": {
409+
"title": "Taxonomic assignment",
410410
"type": "object",
411-
"description": "Options for the DADA2 assignTaxonomy method used to classify ASVs against a database",
412-
"default": "",
413-
"fa_icon": "fas fa-sitemap",
411+
"fa_icon": "fas fa-database",
412+
"description": "Choose a method and database for taxonomic assignments to single-region amplicons",
414413
"properties": {
415414
"dada_min_boot": {
416415
"type": "integer",
417416
"default": 50,
418417
"fa_icon": "fas fa-greater-than-equal",
419-
"description": "The minimum bootstrap confidence (out of 100 trials) for assigning a taxonomic level. Matches `minBoot` in DADA2's assignTaxonomy method.",
418+
"description": "The minimum bootstrap confidence (out of 100 trials) for assigning a taxonomic level with DADA2. Matches `minBoot` in DADA2's assignTaxonomy method.",
420419
"minimum": 0,
421420
"maximum": 100
422-
}
423-
}
424-
},
425-
"taxonomic_database": {
426-
"title": "Taxonomic database",
427-
"type": "object",
428-
"fa_icon": "fas fa-database",
429-
"description": "Choose a method and database for taxonomic assignments to single-region amplicons",
430-
"properties": {
421+
},
431422
"dada_ref_taxonomy": {
432423
"type": "string",
433424
"help_text": "Choose any of the supported databases, and optionally also specify the version. Database and version are separated by an equal sign (`=`, e.g. `silva=138`) . This will download the desired database, format it to produce a file that is compatible with DADA2's assignTaxonomy and another file that is compatible with DADA2's addSpecies.\n\nThe following databases are supported:\n- GTDB - Genome Taxonomy Database - 16S rRNA\n- SBDI-GTDB, a Sativa-vetted version of the GTDB 16S rRNA\n- PR2 - Protist Reference Ribosomal Database - 18S rRNA\n- RDP - Ribosomal Database Project - 16S rRNA\n- SILVA ribosomal RNA gene database project - 16S rRNA\n- UNITE - eukaryotic nuclear ribosomal ITS region - ITS\n- COIDB - eukaryotic Cytochrome Oxidase I (COI) from The Barcode of Life Data System (BOLD) - COI\n\nGenerally, using `gtdb`, `pr2`, `rdp`, `sbdi-gtdb`, `silva`, `coidb`, `unite-fungi`, or `unite-alleuk` will select the most recent supported version.\n\nPlease note that commercial/non-academic entities [require licensing](https://www.arb-silva.de/silva-license-information) for SILVA v132 database (non-default) but not from v138 on (default).",
@@ -1109,10 +1100,7 @@
11091100
"$ref": "#/$defs/asv_post_processing"
11101101
},
11111102
{
1112-
"$ref": "#/$defs/dada2_assigntaxonomy"
1113-
},
1114-
{
1115-
"$ref": "#/$defs/taxonomic_database"
1103+
"$ref": "#/$defs/taxonomic_assignment"
11161104
},
11171105
{
11181106
"$ref": "#/$defs/multiregion_taxonomic_database"

0 commit comments

Comments
 (0)