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@@ -71,11 +71,11 @@ A unique identifier for the ONT sequencing run being demultiplexed.
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Path to the barcode FASTA file used for Dorado demultiplexing. The default barcode file (**barcodes.384.fa**) is located in the `assets` folder.
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#### `--arrangement_toml`
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Path to the TOML configuration file defining the barcode arrangement for Dorado demultiplexing. The default arrangement file (**arrangement.toml**) is located in the assets directory.
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Path to the TOML configuration file defining the barcode arrangement for Dorado demultiplexing. The default arrangement file (**arrangement.toml**) is located in the `assets` folder.
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### Profiles
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Several profiles are available and can be selected with the `-profile` option at the command line. The default profile is `docker`.
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Several profiles are available and can be selected with the `-profile` option at the command line.
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-**test**: Run pipeline using test data.
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-**docker**: Run pipeline using Docker containers.
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```
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output_directory/
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├── demuxed_fastq
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│ ├── barcode001.seqWell.fastq.gz # Final demuxed reads after dorado and cutadapt
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├──demuxed_fastq # demuxed reads after dorado and cutadapt in Nanopore like output structure with subdirectories
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| ├── barcode001
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│ └── barcode001.seqWell.fastq.gz
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|. ├── barcode002
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│ └── barcode002.seqWell.fastq.gz
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|. ├── barcode003
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│ └── barcode003.seqWell.fastq.gz
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| ├── ...
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|. ├── unclassified
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│ └──unclassified.seqWell.fastq.gz
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├── demuxed_fastq_flat
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│ ├── barcode001.seqWell.fastq.gz # demuxed reads after dorado and cutadapt in a flat structure
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