Skip to content

Commit d714752

Browse files
committed
update README.md output section
1 parent 595fb8a commit d714752

1 file changed

Lines changed: 15 additions & 4 deletions

File tree

README.md

Lines changed: 15 additions & 4 deletions
Original file line numberDiff line numberDiff line change
@@ -71,11 +71,11 @@ A unique identifier for the ONT sequencing run being demultiplexed.
7171
Path to the barcode FASTA file used for Dorado demultiplexing. The default barcode file (**barcodes.384.fa**) is located in the `assets` folder.
7272

7373
#### `--arrangement_toml`
74-
Path to the TOML configuration file defining the barcode arrangement for Dorado demultiplexing. The default arrangement file (**arrangement.toml**) is located in the assets directory.
74+
Path to the TOML configuration file defining the barcode arrangement for Dorado demultiplexing. The default arrangement file (**arrangement.toml**) is located in the `assets` folder.
7575

7676
### Profiles
7777

78-
Several profiles are available and can be selected with the `-profile` option at the command line. The default profile is `docker`.
78+
Several profiles are available and can be selected with the `-profile` option at the command line.
7979

8080
- **test**: Run pipeline using test data.
8181
- **docker**: Run pipeline using Docker containers.
@@ -117,12 +117,23 @@ nextflow run \
117117

118118
```
119119
output_directory/
120-
├── demuxed_fastq
121-
│ ├── barcode001.seqWell.fastq.gz # Final demuxed reads after dorado and cutadapt
120+
├──demuxed_fastq # demuxed reads after dorado and cutadapt in Nanopore like output structure with subdirectories
121+
| ├── barcode001
122+
│ └── barcode001.seqWell.fastq.gz
123+
|. ├── barcode002
124+
│ └── barcode002.seqWell.fastq.gz
125+
|. ├── barcode003
126+
│ └── barcode003.seqWell.fastq.gz
127+
| ├── ...
128+
|. ├── unclassified
129+
│ └──unclassified.seqWell.fastq.gz
130+
├── demuxed_fastq_flat
131+
│ ├── barcode001.seqWell.fastq.gz # demuxed reads after dorado and cutadapt in a flat structure
122132
│ ├── barcode002.seqWell.fastq.gz
123133
│ ├── barcode003.seqWell.fastq.gz
124134
│ ├── ...
125135
│ └── unclassified.seqWell.fastq.gz # Unassigned reads
136+
├── barcode008
126137
├── demux_summary/
127138
│ └── demux_report.csv # Demux summary report
128139
├── read_length/

0 commit comments

Comments
 (0)