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‎CHANGELOG.md‎

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- [SeqKit v2.11.0](https://github.com/shenwei356/seqkit/releases/tag/v2.11.0) - 2025-xx-xx
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[![Github Releases (by Release)](https://img.shields.io/github/downloads/shenwei356/seqkit/v2.11.0/total.svg)](https://github.com/shenwei356/seqkit/releases/tag/v2.11.0)
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- [SeqKit v2.10.1](https://github.com/shenwei356/seqkit/releases/tag/v2.10.1) - 2025-08-19
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[![Github Releases (by Release)](https://img.shields.io/github/downloads/shenwei356/seqkit/v2.10.1/total.svg)](https://github.com/shenwei356/seqkit/releases/tag/v2.10.1)
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- `seqkit seq`:
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- fix validing sequences: it failed to report error when the invalid sequence is not the last one in the input. [#536](https://github.com/shenwei356/seqkit/issues/536)
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- `seqkit stats`:

‎doc/docs/download.md‎

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## Current Version
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- [SeqKit v2.10.0](https://github.com/shenwei356/seqkit/releases/tag/v2.10.0) - 2025-03-12
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[![Github Releases (by Release)](https://img.shields.io/github/downloads/shenwei356/seqkit/v2.10.0/total.svg)](https://github.com/shenwei356/seqkit/releases/tag/v2.10.0)
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- `seqkit`:
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- add a global flag `--skip-file-check`: skip input file checking when given a file list if you believe these files do exist.
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It helps to reduce file checking time when given a huge number of sequence files.
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- `seqkit split2`:
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- fix prefix checking when paired-end files are given. [#512](https://github.com/shenwei356/seqkit/issues/512)
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- `seqkit stat`:
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- do not compute GC content and N's for protein sequence. [#497](https://github.com/shenwei356/seqkit/issues/497)
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- `seqkit grep`:
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- add early exit for `--delete-matched` when no patterns remain [#505](https://github.com/shenwei356/seqkit/pull/505) by @sawyerknoblich
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- `seqkit concat`:
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- add an option `-F/--fill` to use a sequence of "-" for IDs missing in some files, can be used in MSA results. [#510](https://github.com/shenwei356/seqkit/issues/510)
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- [SeqKit v2.10.1](https://github.com/shenwei356/seqkit/releases/tag/v2.10.1) - 2025-08-19
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[![Github Releases (by Release)](https://img.shields.io/github/downloads/shenwei356/seqkit/v2.10.1/total.svg)](https://github.com/shenwei356/seqkit/releases/tag/v2.10.1)
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- `seqkit seq`:
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- fix validing sequences: it failed to report error when the invalid sequence is not the last one in the input. [#536](https://github.com/shenwei356/seqkit/issues/536)
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- `seqkit stats`:
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- fix decimal places of some fields when using `-T`.
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- `seqkit fx2tab`:
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- fix the calculation of GC content (`--gc`). Previously, the denominator was the total sequence length, which could lead to inaccuracies due to the potential presence of gaps in the sequence. [#515](https://github.com/shenwei356/seqkit/issues/515)
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- `seqkit sample`:
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- fix `-n` for in-memory mode. [#518](https://github.com/shenwei356/seqkit/issues/518)
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- `seqkit subseq`:
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- fix the bug that subseq --feature is not case insensitive. [#523](https://github.com/shenwei356/seqkit/issues/523)
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- `seqkit grep/locate/mutate`:
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- update help message for `-p/--pattern`, to show how to set multiple values. [#527](https://github.com/shenwei356/seqkit/pull/527) by @corneliusroemer
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### Links
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| OS | Arch | File, 中国镜像 | Download Count |
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| :------ | :--------- | :------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------ | :----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------- |
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| Linux | 32-bit | [seqkit_linux_386.tar.gz](https://github.com/shenwei356/seqkit/releases/download/v2.10.0/seqkit_linux_386.tar.gz), <br/> [中国镜像](http://app.shenwei.me/data/seqkit/seqkit_linux_386.tar.gz) | [![Github Releases (by Asset)](https://img.shields.io/github/downloads/shenwei356/seqkit/latest/seqkit_linux_386.tar.gz.svg?maxAge=3600)](https://github.com/shenwei356/seqkit/releases/download/v2.10.0/seqkit_linux_386.tar.gz) |
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| Linux | **64-bit** | [**seqkit_linux_amd64.tar.gz**](https://github.com/shenwei356/seqkit/releases/download/v2.10.0/seqkit_linux_amd64.tar.gz), <br/> [中国镜像](http://app.shenwei.me/data/seqkit/seqkit_linux_amd64.tar.gz) | [![Github Releases (by Asset)](https://img.shields.io/github/downloads/shenwei356/seqkit/latest/seqkit_linux_amd64.tar.gz.svg?maxAge=3600)](https://github.com/shenwei356/seqkit/releases/download/v2.10.0/seqkit_linux_amd64.tar.gz) |
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| Linux | **arm64** | [**seqkit_linux_arm64.tar.gz**](https://github.com/shenwei356/seqkit/releases/download/v2.10.0/seqkit_linux_arm64.tar.gz), <br/> [中国镜像](http://app.shenwei.me/data/seqkit/seqkit_linux_arm64.tar.gz) | [![Github Releases (by Asset)](https://img.shields.io/github/downloads/shenwei356/seqkit/latest/seqkit_linux_arm64.tar.gz.svg?maxAge=3600)](https://github.com/shenwei356/seqkit/releases/download/v2.10.0/seqkit_linux_arm64.tar.gz) |
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| macOS | **64-bit** | [**seqkit_darwin_amd64.tar.gz**](https://github.com/shenwei356/seqkit/releases/download/v2.10.0/seqkit_darwin_amd64.tar.gz), <br/> [中国镜像](http://app.shenwei.me/data/seqkit/seqkit_darwin_amd64.tar.gz) | [![Github Releases (by Asset)](https://img.shields.io/github/downloads/shenwei356/seqkit/latest/seqkit_darwin_amd64.tar.gz.svg?maxAge=3600)](https://github.com/shenwei356/seqkit/releases/download/v2.10.0/seqkit_darwin_amd64.tar.gz) |
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| macOS | **arm64** | [**seqkit_darwin_arm64.tar.gz**](https://github.com/shenwei356/seqkit/releases/download/v2.10.0/seqkit_darwin_arm64.tar.gz), <br/> [中国镜像](http://app.shenwei.me/data/seqkit/seqkit_darwin_arm64.tar.gz) | [![Github Releases (by Asset)](https://img.shields.io/github/downloads/shenwei356/seqkit/latest/seqkit_darwin_arm64.tar.gz.svg?maxAge=3600)](https://github.com/shenwei356/seqkit/releases/download/v2.10.0/seqkit_darwin_arm64.tar.gz) |
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| Windows | 32-bit | [seqkit_windows_386.exe.tar.gz](https://github.com/shenwei356/seqkit/releases/download/v2.10.0/seqkit_windows_386.exe.tar.gz), <br/> [中国镜像](http://app.shenwei.me/data/seqkit/seqkit_windows_386.exe.tar.gz) | [![Github Releases (by Asset)](https://img.shields.io/github/downloads/shenwei356/seqkit/latest/seqkit_windows_386.exe.tar.gz.svg?maxAge=3600)](https://github.com/shenwei356/seqkit/releases/download/v2.10.0/seqkit_windows_386.exe.tar.gz) |
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| Windows | **64-bit** | [**seqkit_windows_amd64.exe.tar.gz**](https://github.com/shenwei356/seqkit/releases/download/v2.10.0/seqkit_windows_amd64.exe.tar.gz), <br/> [中国镜像](http://app.shenwei.me/data/seqkit/seqkit_windows_amd64.exe.tar.gz) | [![Github Releases (by Asset)](https://img.shields.io/github/downloads/shenwei356/seqkit/latest/seqkit_windows_amd64.exe.tar.gz.svg?maxAge=3600)](https://github.com/shenwei356/seqkit/releases/download/v2.10.0/seqkit_windows_amd64.exe.tar.gz) |
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| Linux | 32-bit | [seqkit_linux_386.tar.gz](https://github.com/shenwei356/seqkit/releases/download/v2.10.1/seqkit_linux_386.tar.gz), <br/> [中国镜像](http://app.shenwei.me/data/seqkit/seqkit_linux_386.tar.gz) | [![Github Releases (by Asset)](https://img.shields.io/github/downloads/shenwei356/seqkit/latest/seqkit_linux_386.tar.gz.svg?maxAge=3600)](https://github.com/shenwei356/seqkit/releases/download/v2.10.1/seqkit_linux_386.tar.gz) |
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| Linux | **64-bit** | [**seqkit_linux_amd64.tar.gz**](https://github.com/shenwei356/seqkit/releases/download/v2.10.1/seqkit_linux_amd64.tar.gz), <br/> [中国镜像](http://app.shenwei.me/data/seqkit/seqkit_linux_amd64.tar.gz) | [![Github Releases (by Asset)](https://img.shields.io/github/downloads/shenwei356/seqkit/latest/seqkit_linux_amd64.tar.gz.svg?maxAge=3600)](https://github.com/shenwei356/seqkit/releases/download/v2.10.1/seqkit_linux_amd64.tar.gz) |
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| Linux | **arm64** | [**seqkit_linux_arm64.tar.gz**](https://github.com/shenwei356/seqkit/releases/download/v2.10.1/seqkit_linux_arm64.tar.gz), <br/> [中国镜像](http://app.shenwei.me/data/seqkit/seqkit_linux_arm64.tar.gz) | [![Github Releases (by Asset)](https://img.shields.io/github/downloads/shenwei356/seqkit/latest/seqkit_linux_arm64.tar.gz.svg?maxAge=3600)](https://github.com/shenwei356/seqkit/releases/download/v2.10.1/seqkit_linux_arm64.tar.gz) |
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| macOS | **64-bit** | [**seqkit_darwin_amd64.tar.gz**](https://github.com/shenwei356/seqkit/releases/download/v2.10.1/seqkit_darwin_amd64.tar.gz), <br/> [中国镜像](http://app.shenwei.me/data/seqkit/seqkit_darwin_amd64.tar.gz) | [![Github Releases (by Asset)](https://img.shields.io/github/downloads/shenwei356/seqkit/latest/seqkit_darwin_amd64.tar.gz.svg?maxAge=3600)](https://github.com/shenwei356/seqkit/releases/download/v2.10.1/seqkit_darwin_amd64.tar.gz) |
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| macOS | **arm64** | [**seqkit_darwin_arm64.tar.gz**](https://github.com/shenwei356/seqkit/releases/download/v2.10.1/seqkit_darwin_arm64.tar.gz), <br/> [中国镜像](http://app.shenwei.me/data/seqkit/seqkit_darwin_arm64.tar.gz) | [![Github Releases (by Asset)](https://img.shields.io/github/downloads/shenwei356/seqkit/latest/seqkit_darwin_arm64.tar.gz.svg?maxAge=3600)](https://github.com/shenwei356/seqkit/releases/download/v2.10.1/seqkit_darwin_arm64.tar.gz) |
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| Windows | 32-bit | [seqkit_windows_386.exe.tar.gz](https://github.com/shenwei356/seqkit/releases/download/v2.10.1/seqkit_windows_386.exe.tar.gz), <br/> [中国镜像](http://app.shenwei.me/data/seqkit/seqkit_windows_386.exe.tar.gz) | [![Github Releases (by Asset)](https://img.shields.io/github/downloads/shenwei356/seqkit/latest/seqkit_windows_386.exe.tar.gz.svg?maxAge=3600)](https://github.com/shenwei356/seqkit/releases/download/v2.10.1/seqkit_windows_386.exe.tar.gz) |
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| Windows | **64-bit** | [**seqkit_windows_amd64.exe.tar.gz**](https://github.com/shenwei356/seqkit/releases/download/v2.10.1/seqkit_windows_amd64.exe.tar.gz), <br/> [中国镜像](http://app.shenwei.me/data/seqkit/seqkit_windows_amd64.exe.tar.gz) | [![Github Releases (by Asset)](https://img.shields.io/github/downloads/shenwei356/seqkit/latest/seqkit_windows_amd64.exe.tar.gz.svg?maxAge=3600)](https://github.com/shenwei356/seqkit/releases/download/v2.10.1/seqkit_windows_amd64.exe.tar.gz) |
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*Notes*
@@ -96,9 +95,9 @@ Run the following commands:
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# ------------------- install golang -----------------
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# download Go from https://go.dev/dl
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wget https://go.dev/dl/go1.24.1.linux-amd64.tar.gz
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wget https://go.dev/dl/go1.25.0.linux-amd64.tar.gz
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tar -zxf go1.24.1.linux-amd64.tar.gz -C $HOME/
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tar -zxf go1.25.0.linux-amd64.tar.gz -C $HOME/
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# or
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# echo "export PATH=$PATH:$HOME/go/bin" >> ~/.bashrc
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cd seqkit/seqkit/
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# optionally choose a release
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# git check v2.10.0
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# git check v2.10.1
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export GOEXPERIMENT=greenteagc # for go1.25
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go build -trimpath -ldflags="-s -w" -tags netgo
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# The executable binary file is located in:
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## Release history
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- [SeqKit v2.10.0](https://github.com/shenwei356/seqkit/releases/tag/v2.10.0) - 2025-03-12
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[![Github Releases (by Release)](https://img.shields.io/github/downloads/shenwei356/seqkit/v2.10.0/total.svg)](https://github.com/shenwei356/seqkit/releases/tag/v2.10.0)
156+
- `seqkit`:
157+
- add a global flag `--skip-file-check`: skip input file checking when given a file list if you believe these files do exist.
158+
It helps to reduce file checking time when given a huge number of sequence files.
159+
- `seqkit split2`:
160+
- fix prefix checking when paired-end files are given. [#512](https://github.com/shenwei356/seqkit/issues/512)
161+
- `seqkit stat`:
162+
- do not compute GC content and N's for protein sequence. [#497](https://github.com/shenwei356/seqkit/issues/497)
163+
- `seqkit grep`:
164+
- add early exit for `--delete-matched` when no patterns remain [#505](https://github.com/shenwei356/seqkit/pull/505) by @sawyerknoblich
165+
- `seqkit concat`:
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- add an option `-F/--fill` to use a sequence of "-" for IDs missing in some files, can be used in MSA results. [#510](https://github.com/shenwei356/seqkit/issues/510)
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- [SeqKit v2.9.0](https://github.com/shenwei356/seqkit/releases/tag/v2.9.0) - 2024-11-01
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[![Github Releases (by Release)](https://img.shields.io/github/downloads/shenwei356/seqkit/v2.9.0/total.svg)](https://github.com/shenwei356/seqkit/releases/tag/v2.9.0)
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- `seqkit`:

‎seqkit/packaging.sh‎

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#!/usr/bin/env sh
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export GOEXPERIMENT=greenteagc # for go1.25
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CGO_ENABLED=0 gox -os="windows darwin linux" -arch="386 amd64 arm64" -tags netgo -ldflags '-w -s' -asmflags '-trimpath'
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dir=binaries

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