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Muliplexed Scanned Temporal Focusing (MuST) and Hybrid Multiplexed Sculpted Light (HyMS) Microscopy

This repository contains mechanical designs, parts lists, processing code, manuals, photos and other resources that enable users skilled in optical and mechanical lab techniques to build the MuST / HyMS microscope as published in the following peer-reviewed article:

Weisenburger, S., Tejera, F., Demas, J., Chen, B., Manley, J., Sparks, F. T., Martínez Traub, F., Daigle, T., Zeng, H., Losonczy, A. & Vaziri, A. Volumetric Ca2+ Imaging in the Mouse Brain Using Hybrid Multiplexed Sculpted Light Microscopy. Cell 177, 1050-1066.e14 (2019). doi:10.1016/j.cell.2019.03.011.

System requirements of the MuST / HyMS processing pipeline

  • MATLAB Base
  • MATLAB Parallel Computing Toolbox
  • MATLAB Image Processing Toolbox
  • MATLAB Statistics and Machine Learning Toolbox
  • ScanImageTiffReader
  • NoRMCorre
  • CaImAn-MATLAB
  • Tested with MATLAB R2017b on Red Hat Enterprise Linux 7.2

For additional information, please refer to the Scan-TeFo / MuST / HyMS dissemination wiki.