no output in freddie_isoform.gtf

[BJ-Chen-Eric]

Hi, thanks for developing the tool. I finish the procedure before freddie_isoform.py and ensure there were output in split, segment and cluster directions. However, when I processed the annotation there is nothing inside the output .gtf. The sample I process is nanopore viral RNA sequening data.

Best wishes

[baraaorabi]

Can you provide me with more details or a sample of your data? Perhaps the reads of one of the larger partitions that result from the Split stage?

[BJ-Chen-Eric]

Hi,
The data I have is viral reads sequenced by nanopore minion direct RNA-seq and the reads is 500k approximately. I will send the original fastq file and the files in split stage to you by the mail on your homepage. Thanks for your help!!