@@ -172,7 +172,7 @@ def bwaalign(bwabin, reffile, inputfile, outfile, threadnumber=1):
172172 inputfile = subprocesspath .subprocesspath (inputfile )
173173 outfile = subprocesspath .subprocesspath (outfile )
174174
175- bwacmd = ' ' .join ([bwabin , 'mem' , '-O' ,' 0' ,' -B' ,' 0' ,' -E' , ' 0' , ' - k' ,' 5' , '-t' ,str (threadnumber ), reffile , inputfile , '>' , outfile ])
175+ bwacmd = ' ' .join ([bwabin , 'mem' , '-O' ,' 0' ,' -B' ,' 0' ,' -k' ,' 5' , '-t' ,str (threadnumber ), reffile , inputfile , '>' , outfile ])
176176
177177 print (bwacmd )
178178
@@ -278,7 +278,7 @@ def bwaloci(bwabin, reffile, inputfile, threadnumber=1):
278278 inputfile = subprocesspath .subprocesspath (inputfile )
279279
280280
281- bwacmd = ' ' .join ([bwabin , 'mem' , '-O' ,' 0' ,' -B' ,' 0' ,' -E' , ' 0' , ' - k' ,' 5' , '-t' ,str (threadnumber ), reffile , inputfile ])
281+ bwacmd = ' ' .join ([bwabin , 'mem' , '-O' ,' 0' ,' -B' ,' 0' ,' -k' ,' 5' , '-t' ,str (threadnumber ), reffile , inputfile ])
282282
283283 print (bwacmd )
284284
@@ -315,7 +315,7 @@ def bwafilter(bwabin, reffile, inputfile, minas, maxxs ,threadnumber=1 ):
315315
316316 inputfile = subprocesspath .subprocesspath (inputfile )
317317
318- bwacmd = ' ' .join ([bwabin , 'mem' , '-O' ,' 0' ,' -B' ,' 0' ,' -E' , ' 0' , ' - k' ,' 5' , '-t' ,str (threadnumber ), reffile , inputfile ])
318+ bwacmd = ' ' .join ([bwabin , 'mem' , '-O' ,' 0' ,' -B' ,' 0' ,' -k' ,' 5' , '-t' ,str (threadnumber ), reffile , inputfile ])
319319
320320 print (bwacmd )
321321
@@ -441,4 +441,4 @@ def bwareflength(bwabin, reffile):
441441 # res = bwaloci(bwapath, '../Test/Testsampe/DM_404.fa', '../Test/Testsampe/DM_test.faprobes.fa',threadnumber=4)
442442 #
443443 # for i in res:
444- # print(i)
444+ # print(i)
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