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umi_tools/prepare_for_em.py

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'''
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==============================================================================
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====================================================================
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prepare_for_em - make the output from dedup compatible with EM tools
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===============================================================================
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prepare_for_rsem - make output from dedup or group compatible with RSEM or
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====================================================================
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prepare_for_em - make output from dedup or group compatible with RSEM or
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Salmon
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Usage: umi_tools prepare_for_rsem [OPTIONS] [--stdin=IN_BAM] [--stdout=OUT_BAM]
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Usage: umi_tools prepare_for_em [OPTIONS] [--stdin=IN_BAM] [--stdout=OUT_BAM]
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note: If --stdout is ommited, standard out is output. To
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generate a valid BAM/SAM/CRAM file on standard out, please
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redirect log with --log=LOGFILE or --log2stderr
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The SAM format specification states that the mnext and mpos fields should point
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to the primary alignment of a read's mate. However, not all aligners adhere to
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this standard.
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prepare-for-em specific options
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-------------------------------
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====================
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--tags=TAG[,TAG....]
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====================
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"""""""""""""""""""""""""
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``--tags =TAG[,TAG....]``
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"""""""""""""""""""""""""
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List of SAM tags that are transfered from read1 to read2. The default
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is UG and BX, which is the numeric UMI group, and the infered true UMI
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respectively.
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'''
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from umi_tools import Utilities as U
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from umi_tools import Documentation
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from collections import defaultdict, Counter
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import pysam
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import sys
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from umi_tools import Documentation
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__doc__ = __doc__ + Documentation.GENERIC_DOCSTRING_SBCRAM_INPUT + Documentation.GENERIC_DOCSTRING_SBCRAM_OUTPUT
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usage = '''
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prepare_for_rsem - make output from dedup or group compatible with RSEM
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prepare_for_em - make output from dedup or group compatible with EM tools
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Usage: umi_tools prepare_for_rsem [OPTIONS] [--stdin=IN_BAM] [--stdout=OUT_BAM]
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Usage: umi_tools prepare_for_em [OPTIONS] [--stdin=IN_BAM] [--stdout=OUT_BAM]
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note: If --stdout is ommited, standard out is output. To
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generate a valid BAM file on standard out, please

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