@@ -5,55 +5,7 @@ These notebooks capture the larger, end-to-end strictosidine case study workflow
55``teemi ``. They are the best starting point if you want to run the published DBTL examples in
66Google Colab with as little setup as possible.
77
8- Each notebook includes a direct Colab launch badge plus a GitHub source link.
9-
10- .. |colab00 | image :: https://colab.research.google.com/assets/colab-badge.svg
11- :alt: Open notebook 00 in Google Colab
12- :target: https://colab.research.google.com/github/hiyama341/teemi/blob/main/colab_notebooks/00_1_DESIGN_Homologs.ipynb
13-
14- .. |colab01 | image :: https://colab.research.google.com/assets/colab-badge.svg
15- :alt: Open notebook 01 in Google Colab
16- :target: https://colab.research.google.com/github/hiyama341/teemi/blob/main/colab_notebooks/01_1_DESIGN_Promoters.ipynb
17-
18- .. |colab02 | image :: https://colab.research.google.com/assets/colab-badge.svg
19- :alt: Open notebook 02 in Google Colab
20- :target: https://colab.research.google.com/github/hiyama341/teemi/blob/main/colab_notebooks/02_1_DESIGN_Combinatorial_library.ipynb
21-
22- .. |colab03 | image :: https://colab.research.google.com/assets/colab-badge.svg
23- :alt: Open notebook 03 in Google Colab
24- :target: https://colab.research.google.com/github/hiyama341/teemi/blob/main/colab_notebooks/03_1_BUILD_gRNA_plasmid.ipynb
25-
26- .. |colab04 | image :: https://colab.research.google.com/assets/colab-badge.svg
27- :alt: Open notebook 04 in Google Colab
28- :target: https://colab.research.google.com/github/hiyama341/teemi/blob/main/colab_notebooks/04_1_BUILD_Background_strain.ipynb
29-
30- .. |colab05 | image :: https://colab.research.google.com/assets/colab-badge.svg
31- :alt: Open notebook 05 in Google Colab
32- :target: https://colab.research.google.com/github/hiyama341/teemi/blob/main/colab_notebooks/05_1_BUILD_Combinatorial_library.ipynb
33-
34- .. |colab06 | image :: https://colab.research.google.com/assets/colab-badge.svg
35- :alt: Open notebook 06 in Google Colab
36- :target: https://colab.research.google.com/github/hiyama341/teemi/blob/main/colab_notebooks/06_1_TEST_Library_characterisation.ipynb
37-
38- .. |colab07 | image :: https://colab.research.google.com/assets/colab-badge.svg
39- :alt: Open notebook 07 in Google Colab
40- :target: https://colab.research.google.com/github/hiyama341/teemi/blob/main/colab_notebooks/07_1_LEARN_Modelling_and_predictions.ipynb
41-
42- .. |colab08 | image :: https://colab.research.google.com/assets/colab-badge.svg
43- :alt: Open notebook 08 in Google Colab
44- :target: https://colab.research.google.com/github/hiyama341/teemi/blob/main/colab_notebooks/08_2_DESIGN_Model_recommended_combinatiorial_library.ipynb
45-
46- .. |colab09 | image :: https://colab.research.google.com/assets/colab-badge.svg
47- :alt: Open notebook 09 in Google Colab
48- :target: https://colab.research.google.com/github/hiyama341/teemi/blob/main/colab_notebooks/09_2_BUILD_Combinatorial_library.ipynb
49-
50- .. |colab10 | image :: https://colab.research.google.com/assets/colab-badge.svg
51- :alt: Open notebook 10 in Google Colab
52- :target: https://colab.research.google.com/github/hiyama341/teemi/blob/main/colab_notebooks/10_2_TEST_Library_characterization.ipynb
53-
54- .. |colab11 | image :: https://colab.research.google.com/assets/colab-badge.svg
55- :alt: Open notebook 11 in Google Colab
56- :target: https://colab.research.google.com/github/hiyama341/teemi/blob/main/colab_notebooks/11_2_LEARN_Modelling_and_predictions.ipynb
8+ Each notebook links to both Google Colab and the GitHub source file.
579
5810Strictosidine Case: First DBTL Cycle
5911------------------------------------
@@ -62,81 +14,69 @@ Design
6214~~~~~~
6315
6416.. list-table ::
65- :widths: 7 43 25 25
17+ :widths: 7 53 40
6618 :header-rows: 1
6719
6820 * - ID
6921 - Focus
70- - Launch
71- - Source
22+ - Links
7223 * - 00
7324 - Automatically fetch homologs from NCBI in a standardizable and repeatable way.
74- - |colab00 |
75- - `GitHub <https://github.com/hiyama341/teemi/blob/main/colab_notebooks/00_1_DESIGN_Homologs.ipynb >`__
25+ - `Open in Colab <https://colab.research.google.com/github/hiyama341/teemi/blob/main/colab_notebooks/00_1_DESIGN_Homologs.ipynb >`__ | `GitHub <https://github.com/hiyama341/teemi/blob/main/colab_notebooks/00_1_DESIGN_Homologs.ipynb >`__
7626 * - 01
7727 - Select promoters from RNA-seq data and fetch them from online databases with quality checks.
78- - |colab01 |
79- - `GitHub <https://github.com/hiyama341/teemi/blob/main/colab_notebooks/01_1_DESIGN_Promoters.ipynb >`__
28+ - `Open in Colab <https://colab.research.google.com/github/hiyama341/teemi/blob/main/colab_notebooks/01_1_DESIGN_Promoters.ipynb >`__ | `GitHub <https://github.com/hiyama341/teemi/blob/main/colab_notebooks/01_1_DESIGN_Promoters.ipynb >`__
8029 * - 02
8130 - Generate a combinatorial library with ``DesignAssembly `` and prepare it for downstream build planning.
82- - |colab02 |
83- - `GitHub <https://github.com/hiyama341/teemi/blob/main/colab_notebooks/02_1_DESIGN_Combinatorial_library.ipynb >`__
31+ - `Open in Colab <https://colab.research.google.com/github/hiyama341/teemi/blob/main/colab_notebooks/02_1_DESIGN_Combinatorial_library.ipynb >`__ | `GitHub <https://github.com/hiyama341/teemi/blob/main/colab_notebooks/02_1_DESIGN_Combinatorial_library.ipynb >`__
8432
8533Build
8634~~~~~
8735
8836.. list-table ::
89- :widths: 7 43 25 25
37+ :widths: 7 53 40
9038 :header-rows: 1
9139
9240 * - ID
9341 - Focus
94- - Launch
95- - Source
42+ - Links
9643 * - 03
9744 - Assemble a CRISPR plasmid with USER cloning.
98- - |colab03 |
99- - `GitHub <https://github.com/hiyama341/teemi/blob/main/colab_notebooks/03_1_BUILD_gRNA_plasmid.ipynb >`__
45+ - `Open in Colab <https://colab.research.google.com/github/hiyama341/teemi/blob/main/colab_notebooks/03_1_BUILD_gRNA_plasmid.ipynb >`__ | `GitHub <https://github.com/hiyama341/teemi/blob/main/colab_notebooks/03_1_BUILD_gRNA_plasmid.ipynb >`__
10046 * - 04
10147 - Construct the background strain by knocking out G8H and CPR.
102- - |colab04 |
103- - `GitHub <https://github.com/hiyama341/teemi/blob/main/colab_notebooks/04_1_BUILD_Background_strain.ipynb >`__
48+ - `Open in Colab <https://colab.research.google.com/github/hiyama341/teemi/blob/main/colab_notebooks/04_1_BUILD_Background_strain.ipynb >`__ | `GitHub <https://github.com/hiyama341/teemi/blob/main/colab_notebooks/04_1_BUILD_Background_strain.ipynb >`__
10449 * - 05
10550 - Generate the first combinatorial library covering 1280 possible combinations.
106- - |colab05 |
107- - `GitHub <https://github.com/hiyama341/teemi/blob/main/colab_notebooks/05_1_BUILD_Combinatorial_library.ipynb >`__
51+ - `Open in Colab <https://colab.research.google.com/github/hiyama341/teemi/blob/main/colab_notebooks/05_1_BUILD_Combinatorial_library.ipynb >`__ | `GitHub <https://github.com/hiyama341/teemi/blob/main/colab_notebooks/05_1_BUILD_Combinatorial_library.ipynb >`__
10852
10953Test
11054~~~~
11155
11256.. list-table ::
113- :widths: 7 43 25 25
57+ :widths: 7 53 40
11458 :header-rows: 1
11559
11660 * - ID
11761 - Focus
118- - Launch
119- - Source
62+ - Links
12063 * - 06
12164 - Process LC-MS data and genotype the generated strains.
122- - |colab06 |
123- - `GitHub <https://github.com/hiyama341/teemi/blob/main/colab_notebooks/06_1_TEST_Library_characterisation.ipynb >`__
65+ - `Open in Colab <https://colab.research.google.com/github/hiyama341/teemi/blob/main/colab_notebooks/06_1_TEST_Library_characterisation.ipynb >`__ | `GitHub <https://github.com/hiyama341/teemi/blob/main/colab_notebooks/06_1_TEST_Library_characterisation.ipynb >`__
12466
12567Learn
12668~~~~~
12769
12870.. list-table ::
129- :widths: 7 43 25 25
71+ :widths: 7 53 40
13072 :header-rows: 1
13173
13274 * - ID
13375 - Focus
134- - Launch
135- - Source
76+ - Links
13677 * - 07
13778 - Use AutoML to predict the best combinations for a targeted second round of library construction.
138- - |colab07 |
139- - `GitHub <https://github.com/hiyama341/teemi/blob/main/colab_notebooks/07_1_LEARN_Modelling_and_predictions.ipynb >`__
79+ - `Open in Colab <https://colab.research.google.com/github/hiyama341/teemi/blob/main/colab_notebooks/07_1_LEARN_Modelling_and_predictions.ipynb >`__ | `GitHub <https://github.com/hiyama341/teemi/blob/main/colab_notebooks/07_1_LEARN_Modelling_and_predictions.ipynb >`__
14080
14181Strictosidine Case: Second DBTL Cycle
14282-------------------------------------
@@ -145,65 +85,57 @@ Design
14585~~~~~~
14686
14787.. list-table ::
148- :widths: 7 43 25 25
88+ :widths: 7 53 40
14989 :header-rows: 1
15090
15191 * - ID
15292 - Focus
153- - Launch
154- - Source
93+ - Links
15594 * - 08
15695 - Translate machine-learning results into a targeted library of strains.
157- - |colab08 |
158- - `GitHub <https://github.com/hiyama341/teemi/blob/main/colab_notebooks/08_2_DESIGN_Model_recommended_combinatiorial_library.ipynb >`__
96+ - `Open in Colab <https://colab.research.google.com/github/hiyama341/teemi/blob/main/colab_notebooks/08_2_DESIGN_Model_recommended_combinatiorial_library.ipynb >`__ | `GitHub <https://github.com/hiyama341/teemi/blob/main/colab_notebooks/08_2_DESIGN_Model_recommended_combinatiorial_library.ipynb >`__
15997
16098Build
16199~~~~~
162100
163101.. list-table ::
164- :widths: 7 43 25 25
102+ :widths: 7 53 40
165103 :header-rows: 1
166104
167105 * - ID
168106 - Focus
169- - Launch
170- - Source
107+ - Links
171108 * - 09
172109 - Construct the targeted second-round library of strains.
173- - |colab09 |
174- - `GitHub <https://github.com/hiyama341/teemi/blob/main/colab_notebooks/09_2_BUILD_Combinatorial_library.ipynb >`__
110+ - `Open in Colab <https://colab.research.google.com/github/hiyama341/teemi/blob/main/colab_notebooks/09_2_BUILD_Combinatorial_library.ipynb >`__ | `GitHub <https://github.com/hiyama341/teemi/blob/main/colab_notebooks/09_2_BUILD_Combinatorial_library.ipynb >`__
175111
176112Test
177113~~~~
178114
179115.. list-table ::
180- :widths: 7 43 25 25
116+ :widths: 7 53 40
181117 :header-rows: 1
182118
183119 * - ID
184120 - Focus
185- - Launch
186- - Source
121+ - Links
187122 * - 10
188123 - Process second-cycle LC-MS characterization data.
189- - |colab10 |
190- - `GitHub <https://github.com/hiyama341/teemi/blob/main/colab_notebooks/10_2_TEST_Library_characterization.ipynb >`__
124+ - `Open in Colab <https://colab.research.google.com/github/hiyama341/teemi/blob/main/colab_notebooks/10_2_TEST_Library_characterization.ipynb >`__ | `GitHub <https://github.com/hiyama341/teemi/blob/main/colab_notebooks/10_2_TEST_Library_characterization.ipynb >`__
191125
192126Learn
193127~~~~~
194128
195129.. list-table ::
196- :widths: 7 43 25 25
130+ :widths: 7 53 40
197131 :header-rows: 1
198132
199133 * - ID
200134 - Focus
201- - Launch
202- - Source
135+ - Links
203136 * - 11
204137 - Run the second ML cycle and inspect improved performance and saturation of top strains.
205- - |colab11 |
206- - `GitHub <https://github.com/hiyama341/teemi/blob/main/colab_notebooks/11_2_LEARN_Modelling_and_predictions.ipynb >`__
138+ - `Open in Colab <https://colab.research.google.com/github/hiyama341/teemi/blob/main/colab_notebooks/11_2_LEARN_Modelling_and_predictions.ipynb >`__ | `GitHub <https://github.com/hiyama341/teemi/blob/main/colab_notebooks/11_2_LEARN_Modelling_and_predictions.ipynb >`__
207139
208140Publication
209141-----------
0 commit comments